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Weekly Updates1 New edition to flow cytometry reference
Practical Flow Cytometry, 4th Edition, is a reference that explains the science and discusses the biomedical applications of quantitative analytical cytology using laser-activated detection and cell sorting. The latest edition has been expanded to provide full coverage of the broad spectrum of applications in molecular biology and biotechnology fields. New to this edition are chapters on automated analysis of array technologies, compensation, high-speed sorting, reporter molecules, and multiplex and apoptosis assays, along with fully updated and revised references and a list of suppliers. Go to www.wiley.com.
2 Free manual for ISAC and CCS members
Representatives from the ISAC international Society for the Advancement of Cytology (ISAC) and Clinical Cytometry Society (CCS) have updated the flow Cytometry. CLIA Compliance Manual There are extensive changes, particularly in the area of quality assessment, making this document useful to flow cytometry laboratories. At the most recent ISAC council meeting, it was decided that this document would be available to registered members of ISAC and/or CCS. After logging onto the ISAC website www.isac-net.org/content/view/784/2/as a member, users can access the document free of charge.
3 Researchers harness nanoparticles to track cancer cell changes
A study, published in April 2009 in the online journal PLoS-ONE, showed that a Stanford University School of Medicine team using specially designed dye-containing nanoparticles was able to simultaneously monitor changes in two intracellular proteins that play crucial roles in the development of cancer. Successful development of the new technique may improve scientists' ability to diagnose cancers by determining how aggressive a tumor's constituent cells are, and eventually separate living cancer cells from one another, based on characteristics indicating their degree of resistance to chemotherapeutic drugs, which would expedite the testing for treatments targeting a tumor's most recalcitrant cells. Visit www.plosone.org.
4 Wiki dedicated to clinical flow cytometry
Clinical Flow Wiki is devoted to building a connected community around clinical flow cytometry. Intended to serve as a resource for information, case examples, e-learning, and resources, the wiki format allows users to share their experience and knowledge by contributing comments, posting sample cases, correcting errors, or adding new topics. Almost every page is editable by registered users. Learn how to participate at wiki.clinicalflow.com.
5 Course uses hands-on and computer work
The 31st annual Clinical Course on Flow and Image Cytometry will be held at the Roswell Park Cancer Institute, Buffalo, NY, from Aug. 30 thru Sept 4, 2009. Emphasis will be divided between basic flow cytometry theory, current practices in the ^^ clinical lab, flow cytometric diagnosis of leukemia and lymphoma, and frontiers for the future. The registration fee is $1,200 {increasing to $1,400 V-S after Aug. 1), and includes course materials. For more information or to register contact Marilyn Price at 716-845-8471 or Marilyn.Price@RoswellPark.org.
6 Expanding cell girth indicates seriousness of breast cancer
The study of how fat cells become after being exposed to a specialized electrical field is helping researchers determine whether cells are normal, cancerous, or a stage cancer already invading other parts of the body. Purdue University scientists tested the electrical process, called microfluidic electroporative flow cytometry, and found that cells that expanded the most were metastatic cancer. The I technique (which has a patent pending) allows screening of single I cells 300 times faster--five cells per second compared with the I one cell per minute of previous methods--which permits testing I of enough cells for diagnosis. See more at www.purdue.edu.
7 Network with industry colleagues
The International Society for Advancement of Cytometry (ISAC) has created online networking groups to help increase communication and information sharing within the cytometry industry. Join ISAC on www.facebook.com and www.linkedin.com. CYT0 2010, ISAC's 25th International Congress, will take place from May 8-12,2010, in Seattle, WA. More information on CYTO 2010 programs will be posted online at www.isac-net.org.
8 New CLSI guidelines provide optimal methods II
CLSI guideline, "Detection of HLA-Specific Alloantibody by Flow Cytometry and Solid Phase Assays; Approved Guideline," describes criteria for optimizing flow cytometry cross matching and the detection of human leukocyte antigen (HLA) alloantibody by solid phase methods in conventional and multiplex platforms. Visit the online store at www.clsi.orgxo purchase the electronic document.
9 Annual CCS meeting
The Clinical Cytometry Society (CCS) will hold the 24th Annual Clinical Cytometry Meeting and Course in Jacksonville, FL, Oct. 16-18 with the CCS course Oct. 19-21. For details, visit www.cytometry.org.
10 MRSA detection
Researchers at the University of Idaho are developing a faster and more accurate method to identify methicillin resistant Staphylococcus aureus (MRSA) strains. Researchers have cut detection time for staph down to three hours and are tweaking the device to allow a complete toxin profile of staph to reveal the virulence of infections. Learn more at www.uidaho.edu.
11 Identify abnormal cells
Testing blood specimens I white blood cells in patients years before the chronic form of lymphocytic leukemia (CLL) develops, according to research published in the New England Journal of Medicine, Feb. 12,2009. The findings may lead to a better understanding of cellular changes that characterize the earliest stages of the disease. For the study, researchers performed a sophisticated form of flow cytometry to identify abnormal B-cell clones. Learn more at content.nejm.org.
12 Book with bonus CD
Flow Cytometry in Clinical Diagnosis, 4th edition, H m offered by the American M T. Society of Clinical Pathologists (ASCP) comes with a CD that includes actual flow analysis and patient samples for 39 cases. The included FCS Reader data analysis software allows users to move gates, change parameters, and evaluate the data and clinical information. Visit the online book store at www.ascp.org.
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